Plasmid Builder v01.10.a

Choose host organism:

Choose Backbone:

Choose the transcription unit:

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Custom CDS

If you need a CDS that is not in the database, you can order synthesis: the insert will be cloned into the selected vector.

5′—

GGTCTC

BsaI

t

AATG

overhang

Your sequence

AGGT

overhang

a

GAGACC

BsaI

—3′

Gene name Sequence

* The sequence must not contain BsaI sites.

Add Multiple Custom CDS

Add multiple custom CDS at once. You can copy-paste data from spreadsheets.

#	Gene Name	Sequence	Actions

0/0 CDS

Account

Profile

Role: —

Name

E-mail

Organisation

Your personal CDS

Here is the list of CDS you added.

Multiple CDS

You can select multiple CDS to add several plasmids to your order with the same general structure but different CDS

Confirm

Add module

Organism Category Level

Module name Module ID (identical to the ID - name from AirTable)

Description(optional) Labels(optional)

Make public (admin only)

Source

.gb file Choose file No file chosen

— OR — sequence (ATGC)

* If you are adding identical modules but they have different sticky ends, they must have the same Module name.

My modules

Edit module

Organism

Category

Level

Module name Module ID (identical to the ID - name from AirTable)

Description(optional) Labels(optional)

Make public (admin only)

* Sequence cannot be changed here.

* If you are adding identical modules but they have different sticky ends, they must have the same Module name.

Public modules

Order Details

Assembly: 0

Synthesis: 0

Assembly

Synthesis

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